Projekte für Studierende

Bachelor-Arbeiten

Initiativbewerbungen bei den Arbeitsgruppenleitern sind gern gesehen und jederzeit möglich.

Master-Arbeiten

Project for master’s thesis in the Kosan lab

“Analysis of T-cell subpopulations in aged wild-type and Miz-1-deficient mice”

Aging leads to reduced immune competence, indicated by a defect in efficient removal of pathogens. In aged mice, remodeling of immune cell populations such as B- and T-cells occurs. Our lab was able to show that the transcription factor Miz-1 is crucial in maintaining B-cell homeostasis and immune competence during aging. However, it is unclear if Miz-1 impairs T‑cell development as well during aging.

To analyze the role of Miz-1 in T cell development we are using a tissue-specific functional knock-out of Miz-1 in mice (loxP/cre-system). Moreover, we have different aged cohorts of wild-type and Miz-1-deficient mice available for analysis. Multi-color flow cytometry is used to identify specific T cell sub populations in primary and secondary lymphoid organs. In addition, we will use cell culture of primary T cells to check for appropriate T cell responses. Additional analyses include measurement of cytokine release, Western blot and qRT-PCR.

We are looking for a highly motivated candidate with basic knowledge in immunology and molecular biology. Interested candidates are invited to send their CV and application to PD Dr. Christian Kosan.

Initiativbewerbungen bei den Arbeitsgruppenleitern sind gern gesehen und jederzeit möglich.